Abstract: In the article in the last issue (Part 1), we discussed the theoretical concept and experimental aspects of nuclear resonant vibrational spectroscopy (NRVS). In this issue, we will focus on its practical applications in iron-specific chemistry and biochemistry. As an introduction, the NRVS techniques for solution and crystal samples of single iron rubredoxin are first described and compared. Then the recent research results of multi-iron iron-sulfur proteins such as ferredoxins,hydrogenases and nitrogenases are discussed in detail. As an example of non-iron-sulfur proteins,carbon monoxide bound myoglobin is also presented. Based on the above examples, the theoretical and experimental advantages and potential of NRVS are reviewed, and its future prospects at home and abroad are discussed.