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纳米分辨率光学显微成像

Nanoscale-resolution optical microscopy

  • 摘要: 光学显微镜发明于17世纪,几百年来其分辨率一直受到光学衍射的限制。进入21世纪,多种超分辨成像技术打破了衍射极限的限制,带来了一场新的显微成像技术革命。其中,基于单分子定位的纳米分辨率光学成像技术近几年更是不断取得突破,达到接近分子尺度的分辨率。文章将简要介绍光学显微镜发展历程及超分辨成像技术基本原理,并着重介绍新型纳米分辨率显微成像技术及应用前景。

     

    Abstract: Optical microscopy, which originated in the 17th century, for two hundred years faced a major limitation due to optical diffraction. However, the 21st century has witnessed a remarkable revolution in microscopic imaging with the introduction of diverse super-resolution techniques designed to surpass this diffraction barrier. Among these, nanoscale resolution optical imaging methods, particularly those centered on single-molecule localization, have consistently achieved groundbreaking results, pushing the boundaries of resolution close to the molecular scale. In this article we provide an overview of the evolution of optical microscopy, delve into the fundamental principles of various super-resolution imaging techniques, and highlight the promising nanoscale resolution microscopy approaches and their potential applications.

     

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